The results of this study demonstrate that CGB scaffolds are biocompatible with hUC-MSCs and that granular CGB scaffolds covered with hUC-MSCs are expected to generate new advances for future treatment of traumatic brain injury.Ĭrown Copyright © 2012. Thus, optimization of chitosan-based scaffolds is clearly required to improved biological and antibacterial properties. BDNF released from CGB scaffold promoted neuronal differentiation of NSCs and led to significant differences in differentiation rate and average neuron perimeter compared with the control group. The CGB scaffolds released BDNF in a uniform profile, whereas the CB scaffolds only released BDNF during the first 3 days. There were on average more cells on the CGB scaffold on the first day than on any other day sampled (P < 0.05). Chitosan scaffolds crosslinked by current methods insufficiently meet the demands of bone tissue engineering applications. There were no significant differences in diameter and porosity of individual CGB scaffolds (P > 0.05). Finally, we investigated whether the released BDNF can induce NSC to differentiate into neurons. The amount of BDNF released from CGB over a 30 day period was determined by ELISA. Prior to this, the diameter and porosity of CGB scaffolds were measured. The viability of hUC-MSCs adhered to the CGB scaffold was determined by digesting with 0.25% trypsin and evaluating with the cell counting kit-8 (CCK-8). The present invention relates to a layered chitosan scaffold wherein said layered scaffold comprises at least two fused layers, wherein at least one of the. Here, we reported a chitosan ink obtained by dissolving chitosan into an alkali aqueous solution. Moreover, the recently reported 3D-printed chitosan scaffolds lack enough strength, thus limiting their use in tissue engineering. Fluorescent nuclear staining (Hoechst 33342) was employed to determine the attachment of the hUC-MSCs to CGB scaffolds on the 1st, 3rd, 7th and 10th day of co-culture. However, chitosan inks are often involved in toxic and organic solvents. Chitosan is considered to be a very promising biopolymer for various biomedical and. hUC-MSCs were co-cultured with the CGB scaffold. Chitosan-Based Scaffolds for Hepatocyte Culture. The chitosan scaffold based on immobilization of BDNF by genipin (GP) as a crosslinking agent referred to hereafter as a CGB scaffold was prepared by freezing-drying technique. This study tested the cytotoxicity of a BDNF blended chitosan scaffold with human umbilical cord mesenchymal stem cells (hUC-MSCs), and the in vitro effect of BDNF blended chitosan scaffolds on neural stem cell differentiation with the aim of contributing alternative methods in tissue engineering for the treatment of traumatic brain injury (TBI).
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